Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. Industry standard disposable (choose from recommended list). 2. The washer has a small footprint and is vacuum and pressure free, The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Cell viability는 세포막 Integrity 또는. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. This integrated system provides users with a multi-detection for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Application Area: Multi mode plate reader. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. These lab workhorses are used in drug discovery, research, bioassay validation, and. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. 9. Description. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Rating: 3. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). Select Start>Control panel>Device Manager. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Shaking: Linear, orbital, and double orbital. 1 fmol luciferase per well. Inquiry Basket. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. Find regional support. Alpha 1536 HTS 96, 384, and . …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. x. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. 5 ng per well. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices,. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. FlexStation 3 Multi Mode Microplate Reader. Microplate is validated by molecular devices for the FLIPR reader. [Carbachol] (nM) RFU Max. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Gemini EM Reader scans. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. As the wells of this plate are closer together, the light is more likely to stray into adjacent wells. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. The budget-friendly SpectraMax® Mini Multi-Mode Microplate Reader maximizes productivity without compromising sensitivity or performance. Analyst, M5, Flex 3, and SpectraMax L readers work differently. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Some have optional microplate readers, allowing emission scans in up to 384-well plates, for example. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. RUO. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Integrated stacker option. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. For nearly 30 years, Molecular Devices has provided scientists with . B. X. • The FlexStation™ II instrument is designed specifically for 96-well microplates. The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. Fluorescence Microplate Reader Comparison Chart. Outer wells are aligned for proper viewing with FLIPR. x. 616, 665 nm EM filters 0200-7011. They perform rapid throughput cell-based assays while. $402. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. After reading the ELISA plate on both readers, each standard curve was nearly identical. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Overview of 384 Well SBS. Molecular Devices: n/a:. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Streamline your workflow with comparative confluence assays to identify and verify gene edits. Download PDF. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. Figure 1. Two different agonists, acetylcholine and carbachol, were tested. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384. 2. Figure 3. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. 0nm increments. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. 0. Visible absorbance -. x. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. , San Diego, CA, USA) such that the. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. Includes PC and software. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. were pipetted into a 96-well black-walled μclear plate. FLIPR Calcium 4 Assay Kit. Category 2 and 3 microplate readers use gradient heating as well. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Versa Max. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. For SpectraMax L or LMax II, LMax II 384 - no adapter used. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Figure 2. Microplate readers can be used to quantify this turbidity to monitor events such as cell growth or to perform solubility screens. It comes standard with 4 wash/rinse bottles and 1 waste bottle for maximum washing flexibility. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. ) 0. SpectraMax Mini Multi-Mode Microplate Reader (Molecular Devices P/N SMAX MINI AF), with fluorescence filter cube FL-535 (Molecular Devices P/N 5089097). or use the modified protocol below for the generation of NPCs (see Note 1). Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Pre-read measurements were made with 200 µL water in each well of a Costar UV plate, after which the plate was emptied and blotted dry. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. The calculated LOQ values were 0. The analyzed results are. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. SpectraMax iD5. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. 6-384 (96- and 384-well with pipetting) 96-well microplates. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. minutes and begin reading microplates with seven mouse clicks. Access this by going to. 20 attomol ATP per well. #PLUS 384) UV-transparent 96-well microplates (Corning cat. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. 384-well plates • Automated rinse reduces clogging • Quiet and efficient. VMax . The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. These advertising cookies will no longer be set for this browser and device. for ELISAs and most cell-based assays. Pro, Dual Read mode was selected. Contact Us Phone: +1-800-635-5577 Web: Email: info@moldev. A ten-fold serial dilution of purified luciferase in PBS with 0. –Min. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. A rinse program is included for quick and easy daily. 7. 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. x. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The SpectraTest validation. 1 fmol luciferase per well. Plus 384. A thermal cycling reaction was immediately initiated. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 057, depending on lot number. Figure 1. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Browse for different fluorescent readers below and compare across different instrument suppliers. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. x. in every plate. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-wellThe FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Molecular Devices Spectramax. 2. Temperature Control: ambient from +4 to 45C. Microplates, cuvettes (via adapter) Detectors. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. More Product Information. 00. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer. For more sample throughput, the system canabsorbance plate readers. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. Not for use in diagnostic procedures. Efficient washing is achieved with cross-wise aspiration, reducing residual volume . All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 190. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. ". 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. Dynamic range > 6 decades . The FLEXstation II 384 (Molecular Devices, Inc. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. We would like to show you a description here but the site won’t allow us. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. +1 800. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 96 deep well plate: packed in sleeves of 6 , 24 per case. Molecular Devices products are warranted to meet the stated specifications. Plus 384. 5 pM 2. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6 to 384 wells, the SpectraMax iD3 reader is the complete solution. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. Our microplate. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. Fluorescence Microplate Reader Comparison Chart. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. There is no product in the shopping cart, buy it!. Reading Capabilities: cuvette or microplate. Automated live cell imaging – cell counting and confluence. Gemini XPS Reader scans. Fluorometers also vary in the size of samples they accept, with some using very small sample sizes to conserve precious sample materials (down to 0. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). Compact design. Industry standard disposable (choose from recommended list). x. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 00. 48 ± 0. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. > Molecular Devices LLC > 384 well plates. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Microplate Formats: 6, 12, 24, 48, 96, 384. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. High base plate design is compatible with automation and for bar coding. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Important things to consider when purchasing a multimode microplate reader. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 96-384. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. VIS absorbance detection. For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Artel MVS Bundle DM Std/800TSNB Reader Details. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. This reader also makes use of a unique temperature. #S1207. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 030 fmol Fluorescence Polarization (FP). Backed by the. 003 fmol 0. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. 340 PC384. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. • 11/10 • Printed in USA • PN: 0120-1525. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. assay volume in a 384-well plate 0200-7017POS Alpha 384 STD 96 and 384-well plates < 100 amol (384-well) 1 0200-7018POS Alpha 384 HTS 96 and 384-well plates < 100 amol (384-well) 1 0200-7019POS Alpha 1536 HTS 96, 384, and 1536-well plates < 100 amol (384-well) 1 HTRF Cisbio HTRF detection with optimized Xenon light source and 616, 665 nm EM filters The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. Round well design with a clear flat bottom 2. Dimensions: 18. 384 wells, the SpectraMax iD3 reader. Validated by Molecular Devices for the FLIPR reader. 76 L x 85. Features. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 384-well format. $542. Time-resolved phosphorescence. Page 1 of 8 . 20 attomol ATP per well. x. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. San Jose, CA 95134. Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. That explains the lower values on the LmaxII384 plate reader. Following the flushing of the machine as described in Section 3. Temperature control. x. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. 0200-6262. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. 1. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. 5in (46. In simplest terms, a plate reader measures and. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. Tb. Figure 1. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. CAPABILITIES OF THE EQUIPMENT: The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring. 1 a. FlexIII. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. High base plate design is compatible with automated handling (gripping) and bar coding. Dual injectors for kinetic assays. Two high-energy xenon flash lamps: Reading capabilities. Integrated stacker option. of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wavelength range (spectral scan. QC tools and IQOQ services. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. and the A1 corner of the plate adapter (Figure 1-2). A. Recertification service. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. 25mm: Plate height:Absorbance was measured at 490 nm with SpectraMax Plus 384 plate reader (Molecular Devices, San Jose, CA). What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Analyst, M5, Flex 3, and SpectraMax L readers work differently. 6-384. Streamline GLP/GMP compliance. Catalog number: BC1384W. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Next generation SpectraMax® absorbance microplate readers. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. 2. This is equivalent to having 511 built-in filters, 384-well: 0. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. If the computer is connected to the Internet: Select Search Automatically for. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. com 上海 电话: 86-21-3372 1088 传真: 86-21-3372. Comprehensive solution. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. ©2010 Molecular Devices, Inc. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. e. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. flat meniscus), but is approx 15 mL. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Microplate Spectrophotometer. 039. 1/17 1907B Printed in USA . X. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. The drawing shows a standard microplate of 15 mm height covered with a lid. 384-well TC plate, Poly-d-Lysine, Flat. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Option 1: Use a plate. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. The SpectraMax ABS Plus reader utilizes Molecular . Four wash/rinse bottles are included and configurable for both 96- and 384. Both readers were heated to 37ºC prior to setting up the assay. Minimum volume and maximum throughput with 96- or. Summary of Contents for Molecular Devices SpectraMax M2. The FilterMax F3 can read 96-well and 384-well plates. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 5577. Plate Formats: 6- to 384-well plates. Total volume (except deep. Another consideration, says Amouretti, is plate format. 4 decades . “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices.